Vol 64 No 4 (2019): Journal of the Chilean Chemical Society
Original Research Papers


Jessy Pavón-Pérez
Universidad de Concepción
Claudia Oviedo
Universidad del Bio Bio
Mhartyn Elso-Freudenberg
Universidad del Bio Bio
Karem Henriquez-Aedo
University of Concepcion
Mario Aranda
Pontificia Universidad Católica de Chile
Published December 18, 2019
  • mass spectrometry,
  • L-DOPA,
  • Vicia faba,
  • LC-MS/MS


A rapid, sensitive, precise and accurate liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for levo 3,4-dihydroxyphenylalanine (L-DOPA) determination in Vicia faba during different growth stages. The method applied a simple sample preparation step followed by a chromatographic separation on a Kinetex XB Core-Shell C18 (100 mm x 4.6 mm, 2.6 µm) column, using a mixture of ultrapure pure water (A) with 0.5 % (v/v) formic acid and methanol (B) as mobile phase. Analysis of L-DOPA was carried out by MS/MS applying a Multiple Reaction Monitoring (MRM) method using the transition m/z 198 → m/z 152. This LC-MS/MS method allowed a well-resolved detection of L-DOPA in ca. 2 min within 6 min run. Method was validated showing a linear range from 0.05 to 10 mg L-1 (R2 = 0.99); repeatability showed RSD value of 1.40%. Recoveries ranged from 94.14 to 116.62% with RSD values ≤ 5.66% and detection and quantification limits were 0.01 and 0.05 mg L-1, respectively. Applying this validated method, L-DOPA was determined in Vicia faba samples to determined its tissue distribution. As expected, a broad range of L-DOPA content finding values from 4.72 (in seeds) up to 133.60 mg g-1 (sprouts).




  1. S. Patil, O. Apine, S. Surwase, J. Jadhav, APD. 2, 7, (2013).
  2. F. Etemadi, M. Hashemi, R. Randhir, O. ZandVakili, A. Ebadi, J. Crop Prod. 6, 426, (2018).
  3. R. Randhir, K. Shetty, Process Biochem. 39, 1775, (2004).
  4. C.M. Gautam M, Azmi W, Ann Phytomed. 1, 1, (2012).
  5. S. Nidhi, Ann. Plant Sci. 4, 1109, (2015).
  6. S. Inamdar, S. Joshi, J. Jadhav, V. Bapat, Nat. prod. bioprospect. 2, 16, (2012).
  7. L. Raguthu, S. Varanese, L. Flancbaum, E. Tayler, A. Di Rocco, Eur J Neurol. 16, 171, (2009).
  8. M. Mehran S M, G. B, J Clin Diagn Res : JCDR. 7, 1004, (2013).
  9. A.P. Raina, R. Khatri, Indian J. Pharm. Sci. 73, 459, (2011).
  10. C. Burbano, C. Cuadrado, M. Muzquiz, J.I. Cubero, Plant Foods Hum Nutr. 47, 265, (1995).
  11. C. Oviedo, M. Elso-Freudenberg, M. Aranda, Appl. Sci. 8, 2431, (2018).
  12. K. Igarashi, K. Hotta, F. Kasuya, K. Abe, S. Sakoda, J. chromatogr. B. 792, 55, (2003).
  13. W. Li, D.T. Rossi, S.T. Fountain, J Pharm Biomed Anal. 24, 325, (2000).
  14. International Conference on Harmonisation (ICH), 2005.
  15. R. Singh, P. Saini, S. Mathur, G. Singh, S. Kumar, Int. J. Green Pharm. 4, 156, (2010).
  16. S. Raghavendra, C.K. Ramesh, V. Kumar, M.H. Moinuddin Khan, Front Life Sci 5, 127, (2011).
  17. C.A. Schenck, H.A. Maeda, Phytochemistry 149, 82, (2018).
  18. C. Goyoaga, C. Burbano, C. Cuadrado, A. Varela, E. Guillamón, M.M. Pedrosa, M. Muzquiz, Eur Food Res Technol. 227, 1537, (2008).

Copyright @2019 | Designed by: Open Journal Systems Chile Logo Open Journal Systems Chile Support OJS, training, DOI, Indexing, Hosting OJS

Code under GNU license: OJS PKP