JOURNAL OF CHILEAN CHEMICAL SOCIETY

Vol 68 No 1 (2023): Journal of the Chilean Chemical Society
Original Research Papers

IN-VITRO EVALUATION OF PHYTOCHEMICALS, HEAVY METALS AND ANTIMICROBIAL ACTIVITIES OF LEAF, STEM AND ROOTS EXTRACTS OF CALTHA PALUSTRIS VAR. ALBA

PDF
  • Syed Wajid Ali Shah,
  • Muhammad Siddique Afridi,
  • Mujaddad Ur Rehman,
  • Azam Hayat,
  • Abid Sarwar,
  • Tariq Aziz,
  • Metab Alharbi,
  • Abdulrahman Alshammari
Syed Wajid Ali Shah
Department of Microbiology Abbottabad University of Science and Technology, Abbottabad, Pakistan
Muhammad Siddique Afridi
Medicinal Botanic Centre, PCSIR Labs Complex, Peshawar 25120, Pakistan
Mujaddad Ur Rehman
Department of Microbiology Abbottabad University of Science and Technology, Abbottabad, Pakistan
Azam Hayat
Department of Microbiology Abbottabad University of Science and Technology, Abbottabad, Pakistan
Abid Sarwar
Food and Biotechnology Research Center, Pakistan Council of Scientific Industrial Research (PCSIR) Lahore, 54600, Pakistan
Tariq Aziz
Department of Agriculture, University of Ioannina, 47100 Arta, Greece
Metab Alharbi
Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Abdulrahman Alshammari
Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Published May 3, 2023
Keywords
  • phytochemicals, antimicrobial activity, heavy metals, Caltha palustris var. alba
How to Cite
Ali Shah, S. W., Afridi, M. S., Rehman, M. U., Hayat, A., Sarwar, A., Aziz, T., Alharbi, M., & Alshammari, A. (2023). IN-VITRO EVALUATION OF PHYTOCHEMICALS, HEAVY METALS AND ANTIMICROBIAL ACTIVITIES OF LEAF, STEM AND ROOTS EXTRACTS OF CALTHA PALUSTRIS VAR. ALBA. Journal of the Chilean Chemical Society, 68(1), 5807-5812. Retrieved from https://www.jcchems.com/index.php/JCCHEMS/article/view/2306

Copyright (c) 2023 SChQ

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Abstract

Plants are a rich source of secondary metabolites that have been found to have medicinal properties. The present study was conducted to evaluate the phytochemical screening, antimicrobial activities, and heavy metal analysis in different parts (leaves, stem and roots) of Caltha palustris var. alba. The phytochemical analysis for the plant was carried out in ethanol, acetone, ethyl acetate, n-hexane and deionized water extracts. The phytochemical screening confirmed the presence of several bioactive compounds like, alkaloids, flavonoids, tannin, phenols, and saponin. The antimicrobial activity was determined by the agar disc diffusion method using five different extracts. The antimicrobial study revealed that the C. palustris var. alba has the ability to fight against the selected microorganism. The phytochemical and biological screenings were correlated with the presence of heavy metals in selected plant C. palustris var. alba. The concentrations of heavy metals were determined by atomic absorption spectrophotometer and revealed the presence of the metals like cadmium, manganese, zinc, copper, lead, and chromium. The metal concentration was compared with the permissible limit set by World Health Organization (WHO) and the result was discussed. From these studies, it is clear that plant C. palustris var. alba can be used for medicinal purposes.

2306.JPG

PDF

References

  1. R. S. Bhat and S. Al-Daihan, "Antimicrobial activity of Garcinia mangostana using different solvents extracts," International Journal of Biosciences, vol. 3, no. 10, pp. 267-272, 2013.
  2. M. Ahmad, M. A. Khan, U. Rashid, M. Zafar, M. Arshad, and S. Sultana, "Quality assurance of herbal drug valerian by chemotaxonomic markers," African Journal of Biotechnology, vol. 8, no. 6, 2009.
  3. S. K. Jain, Manual of ethnobotany. Scientific publishers, 2010.
  4. P. Sheng-Ji, "Ethnobotanical approaches of traditional medicine studies: some experiences from Asia," Pharmaceutical biology, vol. 39, no. sup1, pp. 74-79, 2001.
  5. M. S. Hussain, S. Fareed, M. Saba Ansari, A. Rahman, I. Z. Ahmad, and M. Saeed, "Current approaches toward production of secondary plant metabolites," Journal of pharmacy & bioallied sciences, vol. 4, no. 1, p. 10, 2012.
  6. M. Wink, "Medicinal plants: a source of anti-parasitic secondary metabolites," Molecules, vol. 17, no. 11, pp. 12771-12791, 2012.
  7. M. Ahmad, M. A. Khan, M. Zafar, M. Arshad, S. Sultana, and B. H. Abbasi, "Use of chemotaxonomic markers for misidentified medicinal plants used in traditional medicines," Journal of Medicinal Plants Research, vol. 4, no. 13, pp. 1244-1252, 2010.
  8. B. Casciaro et al., "Nigritanine as a new potential antimicrobial alkaloid for the treatment of Staphylococcus aureus-induced infections," Toxins, vol. 11, no. 9, p. 511, 2019.
  9. R. Khan et al., "Antimicrobial activity of five herbal extracts against multi drug resistant (MDR) strains of bacteria and fungus of clinical origin," Molecules, vol. 14, no. 2, pp. 586-597, 2009.
  10. S. Acharyya, G. K. Dash, S. Mondal, and S. K. Dash, "Antioxidative and antimicrobial study of Spondias mangifera willd root," Int. J. Pharm. Pharm. Sci, vol. 2, no. 4, pp. 68-71, 2010.
  11. J. Hussain et al., "Assessment of herbal products and their composite medicinal plants through proximate and micronutrients analysis," Journal of Medicinal Plants Research, vol. 3, no. 12, pp. 1072-1077, 2009.
  12. E. Ernst, "Toxic heavy metals and undeclared drugs in Asian herbal medicines," Trends in pharmacological sciences, vol. 23, no. 3, pp. 136-139, 2002.
  13. M. Hamayun, "Ethnobotanical profile of Utror and Gabral valleys, district Swat, Pakistan," Ethnobotanical leaflets, vol. 2005, no. 1, p. 9, 2005.
  14. Z. K. Shinwari, "Medicinal plants research in Pakistan," Journal of medicinal plants research, vol. 4, no. 3, pp. 161-176, 2010.
  15. J. T. Williams and Z. Ahmad, "Priorities for medicinal plants research and development in Pakistan," 1999.
  16. C. Walter, Z. K. Shinwari, I. Afzal, and R. N. Malik, "Antibacterial activity in herbal products used in Pakistan," Pak J Bot, vol. 43, pp. 155-62, 2011.
  17. I. Haq, "Antimicrobial agents in Islamic medicine," Hamdard Medicus, vol. 11, no. 4, pp. 496-499, 1997.
  18. Fazal Hadi and Tariq Aziz. A Mini Review on Lead (Pb) Toxicity in Plants. Journal of Biology and Life Science, 6076, 6(2), 91-101.2015
  19. S. M. Freire, L. Torres, N. F. Roque, C. Souccar, and A. J. Lapa, "Analgesic activity of a triterpene isolated from Scoparia dulcis L.(Vassourinha)," Memórias do Instituto Oswaldo Cruz, vol. 86, pp. 149-151, 1991.
  20. C. Garg, S. Khan, S. Ansari, and M. Garg, "Efficacy and safety studies of Foeniculum vulgare through evaluation of toxicological and stadardisation parameters," International Journal of Pharmacy and Pharmaceutical Sciences, vol. 2, no. 2, pp. 43-45, 2010.
  21. I. Baranowska, K. Srogi, A. Wlochowicz, and K. Szczepanik, "Determination of heavy metal contents in samples of medicinal herbs," Polish Journal of Environmental Studies, vol. 11, no. 5, pp. 467-472, 2002.
  22. M. M. Campos et al., "Determination of lead content in medicinal plants by pre‐concentration flow injection analysis–flame atomic absorption spectrometry," Phytochemical Analysis: An International Journal of Plant Chemical and Biochemical Techniques, vol. 20, no. 6, pp. 445-449, 2009.
  23. L. S. Di Toppi and R. Gabbrielli, "Response to cadmium in higher plants," Environmental and experimental botany, vol. 41, no. 2, pp. 105-130, 1999.
  24. H. Ali, M. Nisar, S. Jehandar, and A. Shujaat, "Ethnobotanical study of some elite plants belonging to Dir, Kohistan Valley, Khyber Pukhtunkhwa, Pakistan [J]," Pak J Bot, vol. 43, no. 2, pp. 787-795, 2011.
  25. S. Mubashir, M. Y. Dar, B. A. Lone, M. I. Zargar, and W. A. Shah, "Anthelmintic, antimicrobial, antioxidant and cytotoxic activity of Caltha palustris var. alba Kashmir, India," Chinese journal of natural medicines, vol. 12, no. 8, pp. 567-572, 2014.
  26. E. Omer, S. Hendawy, E. A. Hussein, A. E. Gendy, and A. A. Ezz El-din, "Introduction of Artemisia Species into the Egyptian Cultivation," Journal of Essential Oil Bearing Plants, vol. 19, no. 4, pp. 793-805, 2016.
  27. J. J. Schofield, "Discovering wild plants," Alaska, Western Canada, The Northwest: Alaska Northwest Books Portland, 2000.
  28. N. Seighali, M. Ghomi, S. Zaker, M. Ramezanighara, and P. Karimi, "Medicinal plants diversity in the flora of Langaroud of Iran," Advances in Environmental Biology, pp. 413-418, 2011.
  29. J. Duke and E. Ayensu, "Medicinal Plants ofChina, Reference Publication Inc," ed: CRC Press, Cleveland, 1985.
  30. S. Foster and J. A. Duke, "A field guide to medicinal plants: eastern and central North America," The Peterson field guide series (USA), 1990.
  31. D. E. Moerman, Native american ethnobotany. Timber press Portland, OR, 1998.
  32. J. A. Duke and E. Ayensu, "Medicinal Plants of China. Medicinal Plants of the World. Vol. 1. Algonac, MI," ed: Reference Publications, Inc, 1985.
  33. D. H. S. Gracelin, A. J. De Britto, and P. B. J. R. Kumar, "Antibacterial screening of a few medicinal ferns against antibiotic resistant phyto pathogen," International journal of pharmaceutical sciences and research, vol. 3, no. 3, p. 868, 2012.
  34. R. J. Cannell, "How to approach the isolation of a natural product," in Natural products isolation: Springer, 1998, pp. 1-51.
  35. N. Ullah, I. Ahmad, and S. Ayaz, "In vitro antimicrobial and antiprotozoal activities, phytochemical screening and heavy metals toxicity of different parts of Ballota nigra," BioMed research international, vol. 2014, 2014.
  36. Y. K. Vaghasiya, R. Nair, M. Soni, S. Baluja, and S. Shanda, "Synthesis, structural determination and antibacterial activity of compounds derived from vanillin and 4-aminoantipyrine," Journal of the Serbian Chemical Society, vol. 69, no. 12, pp. 991-998, 2004.
  37. S. Garg, R. Bhushan, and R. Kapoor, "Aervanone, a new flavanone from Aerva persica," Phytochemistry, vol. 19, no. 6, pp. 1265-1267, 1980.
  38. B. Kirby, "Kirby-Bauer Disk Diffusion Susceptibility Test Protocol," American Society for Microbiology, vol. 66, p. 208, 2009.
  39. N. Savithramma, M. L. Rao, and D. Suhrulatha, "Screening of medicinal plants for secondary metabolites," Middle-East Journal of Scientific Research, vol. 8, no. 3, pp. 579-584, 2011.
  40. M. Teja et al., "Preliminary phytochemical evaluation of Caesalpinia sappan saraca indica," Int J Res Pharm Chem, vol. 6, no. 1, 2016.
  41. A. Soni and S. Sosa, "Phytochemical analysis and free radical scavenging potential of herbal and medicinal plant extracts," Journal of Pharmacognosy and Phytochemistry, vol. 2, no. 4, pp. 22-29, 2013.
  42. R. Sheel, K. Nisha, and J. Kumar, "Preliminary phytochemical screening of methanolic extract of Clerodendron infortunatum," IOSR Journal of Applied Chemistry, vol. 7, no. 1, pp. 10-13, 2014.
  43. N. Tamilselvi, P. Krishnamoorthy, R. Dhamotharan, P. Arumugam, and E. Sagadevan, "Analysis of total phenols, total tannins and screening of phytocomponents in Indigofera aspalathoides (Shivanar Vembu) Vahl EX DC," Journal of Chemical and Pharmaceutical Research, vol. 4, no. 6, pp. 3259-3262, 2012.
  44. M. A. Deshmukh and M. A. Theng, "Phytochemical screening, quantitative analysis of primary and secondary metabolites of Acacia arabica bark," International Journal of Current Pharmaceutical Research, vol. 10, no. 2, pp. 35-37, 2018.
  45. E. Charles, "Virtual Chem. Book. Elmhurst. College," ed, 2009.
  46. R. Cook, "Reactions of steroids with acetic anhydride and sulphuric acid (the Liebermann-Burchard test)," Analyst, vol. 86, no. 1023, pp. 373-381, 1961.
  47. S. A. Khan, L. Khan, I. Hussain, K. B. Marwat, and N. Akhtar, "Profile of heavy metals in selected medicinal plants," Pakistan Journal of Weed Science Research, vol. 14, no. 1-2, pp. 101-110, 2008.
  48. A. Ahmed, N. Jahan, A. Wadud, H. Imam, S. Hajera, and A. Bilal, "Physicochemical and biological properties of Adiantum capillus-veneris Linn: an important drug of Unani system of medicine," Int J Cur Res Rev, vol. 4, no. 21, 2012.
  49. M. S. Ishaq, M. M. Hussain, M. Siddique Afridi, G. Ali, M. Khattak, and S. Ahmad, "In vitro phytochemical, antibacterial, and antifungal activities of leaf, stem, and root extracts of Adiantum capillus veneris," The Scientific World Journal, vol. 2014, 2014.
  50. N. Mahmood et al., "Phytochemical screening, antibacterial activity and heavy metal analysis of ethnomedicinal recipes and their sources used against infectious diseases," Plants, vol. 8, no. 11, p. 454, 2019.
  51. V. M. Devi and M. Murugan, "Elemental concentration, phytochemical and antimicrobial activity of Scoparia dulcis Linn," Materials Today: Proceedings, 2021.
  52. I. H. Tipton, P. L. Stewart, and J. Dickson, "Patterns of elemental excretion in long term balance studies," Health physics, vol. 16, no. 4, pp. 455-462, 1969.
  53. Naveed, M.; Batool, H.; Rehman, S.u.; Javed, A.; Makhdoom, S.I.; Aziz, T.; Mohamed, A.A.; Sameeh, M.Y.; Alruways, M.W.; Dablool, A.S.; Almalki, A.A.; Alamri, A.S.; Alhomrani, M. Characterization and Evaluation of the Antioxidant, Antidiabetic, Anti-Inflammatory, and Cytotoxic Activities of Silver Nanoparticles Synthesized Using Brachychiton populneus Leaf Extract. Processes 2022, 10, 1521.
  54. Naveed, M.; Bukhari, B.; Aziz, T.; Zaib, S.; Mansoor, M.A.; Khan, A.A.; Shahzad, M.; Dablool, A.S.; Alruways, M.W.; Almalki, A.A.; Alamri, A.S.; Alhomrani, M. Green Synthesis of Silver Nanoparticles Using the Plant Extract of Acer oblongifolium and Study of Its Antibacterial and Antiproliferative Activity via Mathematical Approaches. Molecules 2022, 27, 4226.
  55. Saleem, A.; Afzal, M.; Naveed, M.; Makhdoom, S.I.; Mazhar, M.; Aziz, T.; Khan, A.A.; Kamal, Z.; Shahzad, M.; Alharbi, M.; Alshammari, A. HPLC, FTIR and GC-MS Analyses of Thymus vulgaris Phytochemicals Executing In Vitro and In Vivo Biological Activities and Effects on COX-1, COX-2 and Gastric Cancer Genes Computationally. Molecules 2022, 27, 8512.
  56. E. Dede and F. Dogara, "The acute toxicological effects of gammalin 20 on the lung and pancreas of guinea pig," Journal of Applied Sciences and Environmental Management, vol. 8, no. 1, pp. 33-35, 2004.
  57. I. Bashiru, "Determination of heavy metals and phytochemical analysis of some selected vegetables grown at Kalambaina Area Sokoto," Asian Journal of Soil Science and Plant Nutrition, pp. 1-7, 2017.
  58. W. Ratnasooriya, J. Jayakody, G. Premakumara, and E. Ediriweera, "Antioxidant activity of water extract of Scoparia dulcis," Fitoterapia, vol. 76, no. 2, pp. 220-222, 2005.
  59. G. Pamunuwa, D. Karunaratne, and V. Y. Waisundara, "Antidiabetic properties, bioactive constituents, and other therapeutic effects of Scoparia dulcis," Evidence-Based Complementary and Alternative Medicine, vol. 2016, 2016.
  60. M. R. Mishra et al., "A brief review on phytoconstituents and ethnopharmacology of Scoparia dulcis Linn.(Scrophulariaceae)," International Journal of Phytomedicine, vol. 3, no. 4, p. 422, 2011.
  61. F. Khuda et al., "Metal analysis, phytotoxic, insecticidal and cytotoxic activities of selected medicinal plants of Khyber Pakhtunkhwa," Pak J Pharm Sci, vol. 25, no. 1, pp. 51-58, 2012.

Copyright @2019 | Designed by: Open Journal Systems Chile Logo Open Journal Systems Chile Support OJS, training, DOI, Indexing, Hosting OJS

Code under GNU license: OJS PKP