JOURNAL OF CHILEAN CHEMICAL SOCIETY

Vol 68 No 2 (2023): Journal of the Chilean Chemical Society
Original Research Papers

ISOLATION, STRUCTURE ELUCIDATION & ANTIDIABETIC POTENTIAL OF ROSA BRUNONII L. FRUIT – FIGHT DIABETES WITH NATURAL REMEDIES

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  • Ejaz Ahmad,
  • Muhammad Jahangeer,
  • Nadeem Irfan Bukhari,
  • Abid Sarwar,
  • Tariq Aziz,
  • Metab Alharbi,
  • Abdulrahman Alshammari,
  • Abdullah F. Alasmari,
  • Abid Sarwar
Ejaz Ahmad
Department of Chemistry, GC University Lahore, Pakistan.
Muhammad Jahangeer
Department of Chemistry, GC University Lahore, Pakistan.
Nadeem Irfan Bukhari
Punjab University College of Pharmacy, University of the Punjab, Lahore, Pakistan.
Abid Sarwar
Food and Biotechnology Research Center, PCSIR Labs Complex Lahore 54600, Punjab Pakistan
Tariq Aziz
Department of Agriculture, University of Ioannina, 47100 Arta, Greece
Metab Alharbi
Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Abdulrahman Alshammari
Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Abdullah F. Alasmari
Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Abid Sarwar
Food and Biotechnology Research Center, PCSIR Labs Complex Lahore 54600, Punjab Pakistan
Published August 22, 2023
Keywords
  • Anti-diabetic activity, alpha-amylase inhibition activity, alpha-glucosidase inhibition activity, Rosa brunonii L. fruit, Catechin
How to Cite
Ahmad, E., Jahangeer, M., Irfan Bukhari, N., Sarwar, A., Aziz, T., Alharbi, M., Alshammari, A., Alasmari, A. F., & Sarwar, A. (2023). ISOLATION, STRUCTURE ELUCIDATION & ANTIDIABETIC POTENTIAL OF ROSA BRUNONII L. FRUIT – FIGHT DIABETES WITH NATURAL REMEDIES. Journal of the Chilean Chemical Society, 68(2), 5887-5894. Retrieved from https://www.jcchems.com/index.php/JCCHEMS/article/view/2355

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Abstract

Diabetes mellitus (DM) is a metabolic disorder that is marked by high blood glucose levels. Members of the Rosaceae family are a good source of antioxidants. Therefore, the current work sought to examine the potential for in-vitro alpha-amylase and alpha-glucosidase inhibition and the antidiabetic activity of Rosa brunonii L. fruit chloroform extract (RBFCE) against Alloxan (ALXN) induced diabetes in rats. RBFCE concentrations ranging from 20, 50, 100, 250, 500, and 750 g/mL were used in in-vitro activities, while oral doses of 500 mg/kg, 750 mg/kg, and 1000 mg/kg were given to rats in an in-vivo trial for 21 days. Isolation was carried out through column chromatography and modern spectroscopic techniques were used for characterization and structure elucidation. The isolated compound was identified as catechin. For alpha-amylase and alpha-glucosidase inhibition activity, RBFCE had IC50 values of 322.06±17.40 and 248.93±1.62, respectively. The IC50 values for acarbose against alpha-amylase and alpha-glucosidase inhibition were 64.64±3.70 and 67.60±4.20, respectively. RBFCE treatment regulated blood glucose levels dose-dependently over a 21-day study period. Histopathological studies revealed that RBFCE has recovered the damaged acinar structures to some extent in pancreatic tissue. Only focal tissue destruction observed. RBFCE treatment displayed normal glomeruli with no signs of inflammation, proliferation, necrosis, cipher of thyroidization and fibrosis. All the extract-treated groups had more protected pancreatic and kidney tissue than control group in dose-dependent manner. The current study results revealed that RBFCE had prominent alpha-amylase and alpha-glucosidase inhibition activity, regulates blood glucose level and normalize histopathological markers in diabetic rats compared to the negative control group.

 

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