JOURNAL OF CHILEAN CHEMICAL SOCIETY

Vol 70 No 1 (2025): Journal of The Chilean Chemical Society
Original Research Papers

BIOCHEMICAL DIVERSITY AND COMPOSITION OF THE UNSTUDIED PATAGONIAN ENDEMIC PLANT Benthamiella azorella (skottsb). Soriano: ANTIOXIDANT, ANTIBACTERIAL AND CYTOTOXIC ACTIVITY ON VARIOUS HUMAN CELL LINES

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  • Dafne Díaz-Hernández,
  • Borja Martinez-Albardonedo,
  • Salvador Arijo,
  • Víctor Sanhueza,
  • José Becerra,
  • Roberto T. Abdala-Díaz,
  • Victor Fajardo
Published September 5, 2025
Keywords
  • Benthamiella,
  • Patagonia,
  • Antioxidant,
  • Antibacterial,
  • Cytotoxicity,
  • Polysaccharides,
  • LC-MS,
  • Metabolites profiles
    • ...More
    Less
How to Cite
Díaz-Hernández, D., Martinez-Albardonedo, B., Arijo, S., Sanhueza, V., Becerra, J., Abdala-Díaz, R. T., & Fajardo, V. (2025). BIOCHEMICAL DIVERSITY AND COMPOSITION OF THE UNSTUDIED PATAGONIAN ENDEMIC PLANT Benthamiella azorella (skottsb). Soriano: ANTIOXIDANT, ANTIBACTERIAL AND CYTOTOXIC ACTIVITY ON VARIOUS HUMAN CELL LINES. Journal of the Chilean Chemical Society, 70(1), 6250-6261. Retrieved from https://www.jcchems.com/index.php/JCCHEMS/article/view/2883

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Abstract

Benthamiella (Solanaceae) is an unstudied endemic genus of the Chilean-Argentinean Patagonia that thrives in harsh climatic and geographic conditions. This study provides a biochemical description of the aerial parts, roots, and polysaccharides of B. azorella, and evaluates their antioxidant, antiproliferative and antibacterial activity. GC-MSanalysis of B. azorella roots polysaccharides identified significant amounts of arabinose, glucose and galacturonic acid. The FT-IR spectrum revealed a diverse range of functional groups. Both analyses suggest a complex polysaccharide structure that may enhance the sample’s functional properties. Elemental analysis showed low nitrogenand sulfur content, while proximate analysis showed significant differences in carbohydrates, lipids, fiber, and ash content between the plant parts. Polyphenols quantification determined a higher concentration in the roots (6.66 ± 0.62 mg GAE g-1 DW) compared to the aerial parts. Likewise, the highest antioxidant capacity was observed in theroots using the DPPH (89.43 ± 0.74 µmol AAE g-1 DW at 1818 µg mL-1) method. The aqueous root extract exhibited higher activity against colon cancer HCT-116 followedby aerial parts. Polysaccharides showed slight activity against hepatocytes cancer HepG2. The extracts behaved variably on the healthy keratinocytes HACAT cell line, tending to promote cell proliferation. Both, aqueous and ethanolic B. azorella solutions were non-toxic, did not show quorum quenching and antibacterial activity against human and fish bacterial strains at the tested concentrations. Finally, 21 metabolites, principally hydroxycoumarins, sapogenins and steroids derivatives were tentatively identified in themost active extract using LC-MS analysis. Further assays of B. azorella roots with cancer and healthy cells lines and new bacterial analysis at higher concentrations are recommended.

 

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