JOURNAL OF CHILEAN CHEMICAL SOCIETY

Vol 61 No 1 (2016): Journal of the Chilean Chemical Society
Original Research Papers

TEMPERATURE EFFECT ON BINDING AFFINITY AND STOICHIOMETRY BETWEEN SOME STEROIDS AND HUMAN SERUM ALBUMIN

Serkan Özdemir
Department of Chemistry, Faculty of Arts and Sciences, Ondokuz Mayıs University, 55139, Atakum, Samsun
Ender Biçer
Department of Chemistry, Faculty of Arts and Sciences, Ondokuz Mayıs University, 55139, Atakum, Samsun
Published December 10, 2015
Keywords
  • Steroids,
  • Human serum albumin,
  • Binding affinity,
  • Temperature effect,
  • Voltammetry
How to Cite
Özdemir, S., & Biçer, E. (2015). TEMPERATURE EFFECT ON BINDING AFFINITY AND STOICHIOMETRY BETWEEN SOME STEROIDS AND HUMAN SERUM ALBUMIN. Journal of the Chilean Chemical Society, 61(1). Retrieved from https://www.jcchems.com/index.php/JCCHEMS/article/view/49

Abstract

The temperature dependence of the interactions between some steroids (fusidic acid, ouabain and ethynodioldiacetate) and human serum albumin (HSA) at physiological pH (7.4) were studied systematically by square-wave voltammetry. Also, these interactions were proven by means of UV–Vis. absorption spectroscopy and FT-IR spectroscopy techniques. The interaction parameters (binding constants and stoichiometries) at temperature range of 297.5-312.5 K were determined from electrochemical data. It is worthy that the temperature has played a positive role on the interactions of fusidic acid with HSA; however it has negative effective on the interaction parameters of HSA with ouabain or ethynodioldiacetate.

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