JOURNAL OF CHILEAN CHEMICAL SOCIETY

Vol 63 No 1 (2018): Journal of the Chilean Chemical Society
Original Research Papers

STUDY OF DNA–DIETHYLSTILBOESTROL INTERACTION USING GRAPHENE-BASED THIN-FILM SENSOR AND UV–VIS SPECTROSCOPY

PDF
  • Meifeng Chen,
  • Xinying Ma,
  • Xia Li,
  • Mingjing Yin,
  • Yanyun Li,
  • Zhanghua Sun
Meifeng Chen
School of Chemistry and Chemical Engineering, Heze University, Heze
Xinying Ma
School of Chemistry and Chemical Engineering, Heze University, Heze
Xia Li
School of Chemistry and Chemical Engineering, Heze University, Heze
Mingjing Yin
School of Chemistry and Chemical Engineering, Heze University, Heze
Yanyun Li
School of Chemistry and Chemical Engineering, Heze University, Heze
Zhanghua Sun
Environmental monitoring central station of Heze, Heze.
Published April 25, 2018
Keywords
  • diethylstilboestrol,
  • DNA,
  • interaction,
  • electrochemical method,
  • UV–Vis spectroscopy
How to Cite
Chen, M., Ma, X., Li, X., Yin, M., Li, Y., & Sun, Z. (2018). STUDY OF DNA–DIETHYLSTILBOESTROL INTERACTION USING GRAPHENE-BASED THIN-FILM SENSOR AND UV–VIS SPECTROSCOPY. Journal of the Chilean Chemical Society, 63(1). Retrieved from https://www.jcchems.com/index.php/JCCHEMS/article/view/574

Copyright (c) 2018 Journal of the Chilean Chemical Society

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Abstract

The graphene-modified glassy carbon electrode (Gr/GCE) was fabricated by casting graphene (Gr). Some electrochemical parameters for diethylstilbestrol (DES) detection, such as pH, scan rate, and accumulation time were discussed. The interaction of DES with DNA at a Gr/GCE was studied by electrochemical method and factors influencing the interaction were optimized. Under the optimized conditions, the results revealed that the peak current decreased and the peak potential shifted to the positive side after adding DNA into the solution containing DES. We deduced that the interaction of DES with DNA mainly is intercalation. The interaction of DES with DNA was studied by ultraviolet and visible (UV-Vis) absorption spectroscopy. Also, with the addition of DNA, hyperchromic effect and small red shifts were observed in its UV-VIS spectra upon addition of DNA, results indicated that the DES molecule intercalated into the DNA, and the complex of DES-DNA was formed.

PDF

References

  1. P. White, H. Hunt, J. Clin. Endocrinol. 3, 500, (1943).
  2. A. L. Herbst, H. Ulfelder, D. C. Poskanzer, The New England Journal of Medicine 284, 878, (1971).
  3. L. Titus-Ernstoff, E. E.Hatch, R. N. Hoover, J. Palmer, E. R. Greenberg, W. Ricker, R. Kaufman, K. Noller, A. L. Herbst, T. Colton, P. Hartge, Br. J. Cancer. 84, 126, (2001).
  4. L. Titus-Ernstoff, R. Troisi, E. E. Hatch, J. R. Palmer, L. A. Wise, W. Ricker, M. Hyer, R. Kaufman, K. Noller, W. Strohsnitter, A. L.Herbst, P. Hartge, R. N. Hoover, Br. J. Cancer., 95,107, (2006).
  5. J. B. Quintana, J.Carpinteiro, I. Rodríguez, R. A. Lorenzo, A. M. Carro, R. Cela, J. Chromatogr. A 1024, 177, (2004).
  6. A. Prieto, A. Vallejo, O. Zuloaga, A. Paschke, B. Sellergen, E. Schillinger, S. Schrader, M. Moder, Anal. Chim. Acta 703, 41, (2011).
  7. K. Sanfilippo, B. Pinto, M. P. Colombini, U. Bartolucci, D. Reali, J. Chromatogr. B 878,1190, (2010).
  8. X.P He, X.Q Mei , J.T Wang , , Z.L, L.J Tan , W. Wu, Mar. Pollut. Bull. 102, 142–147, (2016).
  9. Q. Xu, M. Wang, S. Q. Yu, Q. Tao, M. Tang, Analyst 136,5030, (2011).
  10. S. Rodriguez-Mozaz, M. J. Lopez de Alda, D. Barcelo, Anal. Chem. 76, 6998, (2004).
  11. X. B. Chen, Y. L. Wu, T. Yang, J. Chromatogr. B, 879,799, (2011).
  12. K. Mitani, M. Fujioka, H. Kataoka, J. Chromatogr. A 1081, 218, (2005).
  14. S. Liu, Q. Lin, X. M. Zhang, X. R. He, X. R. Xing, W. J. Lian, J. Li, M. Cui, J. D. Huang, Sens. Actuators B, 166/167, 562, (2012).
  15. Q. L. Zhang, J. Li, T. T Ma, Z. T. Zhang, Food Chem.111, 498, (2008).
  16. J. J. Fei, X. Q. Wen, L. H. Yi, F. Ge, Y. Zhang, M. H. Huang, X. M.Chen, J. Appl. Electrochem. 38, 1527, (2008).
  17. C. M. Yu, W. Y. Ji, Y. D. Wang, N. Bao, H. Y. Gu, Nanotechnology 241, 15502, (2013).
  18. K. M. Qu, X. Z. Zhang, Z. L. Lv, M. Li, Z. G. Cui, Y. Zhang, B. J. Chen, S. S. Ma, Q. Kong, Int. J. Electrochem. Sci. 7, 1827, (2012).
  19. X. Y. Ma, M. F.Chen, Sens. Actuators B 215, 445, (2015).
  20. L.T Hu, Q. Cheng, D.C. Chen , M. Ma , K.B. Wu, J. Hazard. Mater. 283,157–163, (2015).
  21. X. L. Wang, J. J. Li, T. Wang, Z. Y. Yu, Ionics 21,1105, (2015).
  22. F. Li, J. Q. Li, Y. Feng, L. M. Yang, Z. F. Du, Sensor Actuat. B-Chem. 157, 110, (2011).
  23. Y. R. Kim, S. Bong, Y. J. Kang, Y. Yang, R. K. Mahajan, J. S. Kim, H. Kim, Biosens. Bioelectron. 25, 2366, (2010).
  24. M. F. Chen, X. Y. Ma, X. Li, J. Solid State Electrochem.16, 3261, (2012).
  25. X.Y. Ma, M. Y. Chao, M. F. Chen, Russ. J. Electrochem. 50, 154, (2014).
  26. B. X. Ye, C. H. Wang, A. H. Jing, J. Chin. Chem. Soc. 50, 457, (2003).
  27. M. T. Carter, M. Rodriguez, A. J. Bard, J. Am. Chem. Soc.111, 8901, (1989).
  28. L. Fotouhi, A.B. Hashkavayi, and M.M. Heravi, Int. J. Bio.l Macromo. 53,101, (2013).
  29. M. Aslanoglu, Anal, Sci. 22, 439, (2006).
  30. C. V. Kumar, E. H. Asuncion, J. Am. Chem. Soc. 115, 8541, (1993).
  31. A. M. Nowicka, E. Zabost, M. Donten, Z. Mazerska, Z. Stojek, Bioelectrochemistry, 70, 440, (2007).
  33. Y. N. Ni, M. Wei, S. Kokot, Int. J. Biol.Macromol. 49, 622, (2011).

Copyright @2019 | Designed by: Open Journal Systems Chile Logo Open Journal Systems Chile Support OJS, training, DOI, Indexing, Hosting OJS

Code under GNU license: OJS PKP