JOURNAL OF CHILEAN CHEMICAL SOCIETY

Vol 63 No 4 (2018): Journal of the Chilean Chemical Society
Original Research Papers

EVALUATION OF MONOLITHIC COLUMN FOR INORGANIC MERCURY AND METHYLMERCURY DETERMINATION IN FISH SAMPLE ANALYSIS

Manuel A. Bravo
Laboratorio de Química Analítica y Ambiental, Pontificia Universidad Católica de Valparaíso
Ornella Riquelme
Laboratorio de Química Analítica y Ambiental, Pontificia Universidad Católica de Valparaíso
Sonnia Parra
Laboratorio de Química Analítica y Ambiental, Pontificia Universidad Católica de Valparaíso
Jan Bergmann
Laboratorio de Química Analítica y Ambiental, Pontificia Universidad Católica de Valparaíso
Waldo Quiroz
Laboratorio de Química Analítica y Ambiental, Pontificia Universidad Católica de Valparaíso
Published January 9, 2019
Keywords
  • Mercury speciation,
  • Monolithic column,
  • Fish samples
How to Cite
Bravo, M. A., Riquelme, O., Parra, S., Bergmann, J., & Quiroz, W. (2019). EVALUATION OF MONOLITHIC COLUMN FOR INORGANIC MERCURY AND METHYLMERCURY DETERMINATION IN FISH SAMPLE ANALYSIS. Journal of the Chilean Chemical Society, 63(4). Retrieved from https://www.jcchems.com/index.php/JCCHEMS/article/view/924

Abstract

In the present work, the applicability of a monolithic column was evaluated for mercury speciation analysis in certified and real fish samples. The influence of the flow and composition of the mobile phase (2-mercaptoethanol concentration and methanol content) on the retention times of iHg and MeHg was evaluated. The optimal separation conditions were achieved for a mobile phase containing 5 % methanol and 0.02 % mercaptoethanol in acetate buffer (pH 5.0) at a flow rate of 1.6 ml min-1. Different extraction conditions were studied to analyze a certified reference material with the optimized methodology and the best results (93 % recovery) were obtained for microwave-assisted extraction with a mixture of HCl 6 M + NaCl 0.5 M. Finally, the proposed method was satisfactorily applied to real fish samples collected from different markets in the central region of Chile, presenting levels ranging from 0.73 – 3.4 mg kg-1.

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